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| Registros recuperados: 237 | |
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| Monfort, Patrick; Hervio Heath, Dominique; Caprais, Marie-paule; Pommepuy, Monique; Annezo, Jean-pierre; Loaec, Solen; Le Mennec, Cecile; Guillerm, Emmanuelle; Boulben, Sylviane; Bilien, Gwenael; Bonsor, Robert; Porter, Jonathan; Pickup, Roger. |
| Le projet européen Cycleau, initié à l'échelle locale sur la problèmatique de l'envasement et de l'ensablement de l'estuaire du Bélon, y a également intégré un volet bactériologique en raison des contraintes fortes que faisaient peser les pics conjoncturels de contamination sur la profession conchylicole (fermetures temporaires, déclassement éventuel) mais plus largement sur l'image du territoire (impact touristique) et en définitive sur son développement durable. Pour satisfaire la restauration de la qualité des eaux estuariennes, l'IFREMER a fait appel à un certain nombre d'outils susceptibles de contribuer à la formulation d'un diagnostic pertinent et ainsi de répondre aux préoccupations des acteurs du littoral. Pour ce faire, nous avons créé un réseau... |
| Tipo: Text |
Palavras-chave: Bassin versant; Contamination fécale; Escherichia coli; Flux bactériens; Bactériophages; SIG; Évaluation. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00200/31077/29490.pdf |
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| Piquet, Jean-come. |
| Tout coquillage mis en vente est issu d’une zone de production (gisement naturel ou concession) classée et surveillée au niveau sanitaire. Quatre classements sont définis de A à D par ordre décroissant de salubrité, du classement A (bonne qualité) autorisant la commercialisation directe des coquillages, au classement D (très mauvaise qualité). Pour définir ce classement, une étude sanitaire est préalablement conduite par l’Ifremer sur demande de l’Administration. L’étude sanitaire réalisée à Bonne Anse en Charente-Maritime, a abouti en 2009 à un arrêté préfectoral classant en B le gisement de palourdes. Aujourd’hui, la qualité sanitaire de la zone est surveillée régulièrement par l’Ifremer, et environ 25 pêcheurs à pied professionnels exploitent le gisement. |
| Tipo: Text |
Palavras-chave: Etude sanitaire; Pêche à pied professionnelle; Charente-Maritime; Bonne Anse; Escherichia coli; Cadmium. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00048/15965/13405.pdf |
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| Barboza,L.L.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.; Fonseca,A.S.. |
| Semiconductor laser devices are readily available and practical radiation sources providing wavelength tenability and high monochromaticity. Low-intensity red and near-infrared lasers are considered safe for use in clinical applications. However, adverse effects can occur via free radical generation, and the biological effects of these lasers from unusually high fluences or high doses have not yet been evaluated. Here, we evaluated the survival, filamentation induction and morphology of Escherichia coli cells deficient in repair of oxidative DNA lesions when exposed to low-intensity red and infrared lasers at unusually high fluences. Cultures of wild-type (AB1157), endonuclease III-deficient (JW1625-1), and endonuclease IV-deficient (JW2146-1) E. coli, in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA; Escherichia coli; Filamentation; Laser. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000013 |
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| Canuto,K.S.; Sergio,L.P.S.; Guimarães,O.R.; Geller,M.; Paoli,F.; Fonseca,A.S.. |
| Low-level lasers are used at low power densities and doses according to clinical protocols supplied with laser devices or based on professional practice. Although use of these lasers is increasing in many countries, the molecular mechanisms involved in effects of low-level lasers, mainly on DNA, are controversial. In this study, we evaluated the effects of low-level red lasers on survival, filamentation, and morphology of Escherichia colicells that were exposed to ultraviolet C (UVC) radiation. Exponential and stationary wild-type and uvrA-deficient E. coli cells were exposed to a low-level red laser and in sequence to UVC radiation. Bacterial survival was evaluated to determine the laser protection factor (ratio between the number of viable cells after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA; Escherichia coli; Laser; Ultraviolet radiation. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000012 |
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| Gonzalez,Ramon. |
| Metabolic engineering was formally defined more than two decades ago (Bailey, 1991) and it is now an established discipline. Metabolic engineering is generally defined as the directed improvement of product formation or cellular properties through the modification of specific biochemical reactions or the introduction of new ones with the use of recombinant DNA technology (Bailey, 1991; Stephanopoulos et al. 1998). Therefore, the analysis and engineering/synthesis of metabolic pathways is of central importance to metabolic engineering. The analytical part uses a number of experimental and modeling techniques for the systematic study of cellular responses (in terms of RNA, protein and metabolite levels, metabolic fluxes, etc.) to genetic and environmental... |
| Tipo: Journal article |
Palavras-chave: Biofuels; Escherichia coli; Metabolic engineering; Systems biology. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300017 |
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| Loguercio,Andrea Pinto; Aleixo,José Antônio Guimarães. |
| Com o objetivo de avaliar as condições higiênico-sanitárias do queijo Minas Frescal produzido artesanalmente em Cuiabá - MT, foi realizada análise microbiológica de trinta amostras obtidas em dois pontos de comercialização. A análise microbiológica consistiu da contagem em placas de Staphylococcus aureus e da contagem estimativa de coliformes totais, fecais e Escherichia coli pela técnica do NMP-3 tubos. Na determinação de coliformes fecais, 28 amostras (93,33%) apresentaram número mais provável (NMP) > 10² NMP/g e somente duas amostras (6,67%) estavam dentro dos padrões legais exigidos. Na contagem de S. aureus, em 29 amostras (96,67%) obteve-se valores superiores a 10³ufc/g, estando apenas 1 amostra (3,33%) em conformidade com o padrão legal.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Queijo Minas Frescal; Coliformes; Staphylococcus aureus; Escherichia coli. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000600024 |
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| Machado,Débora Cabral; Maia,Carla Marques; Carvalho,Isabel Dias; Silva,Natan Fontoura da; André,Maria Cláudia Dantas Porfírio Borges; Serafini,Álvaro Bisol. |
| An attempt was made to evaluate microbiological quality of horticultural crops grown organically. Three species of vegetables were used, lettuce (Lactuva sativa), radish (Raphanus sativus) and spinach (Tetragonia expansa), grown organically, in fertile soil. Six different treatments were applied: mineral fertilizer, chicken, cow, and pig manure, chicken litter and cow manure, in association with a liquid foliar biofertilizer. These crops were handled according to correct agronomic practices for growing crops organically for commercial purposes. Samples were examined for the Most Probable Number (MPN/g/mL) of total and fecal coliforms and to detect the presence of Escherichia coli and Salmonella spp. All analyzed samples were considered acceptable for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Organic vegetables; Animal manure; Coliforms; Escherichia coli; Salmonella. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400025 |
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| Pereira,Murilo Anderson; Nunes,Márcia Menezes; Nuernberg,Leonardo; Schulz,Denys; Batista,Cleide Rosana Vieira. |
| Oysters are filter feeders able to ingest particles in suspension that may carry pathogenic microorganisms. In this respect, the consumption of raw oysters can cause foodborne diseases in humans. The aim of the present study was to evaluate the microbiological quality of Crassostrea gigas oysters cultivated and commercialized in the coastal region of Florianópolis, SC, Brazil. The study comprised counts of coliforms at 35ºC and at 45ºC, Escherichia coli and coagulase-positive staphylococci, and detection of Salmonella sp, Vibrio cholerae and Vibrio parahaemolyticus. Ninety samples were analyzed, 45 of them collected at seafood commercial establishments and the other 45 were collected in the cultivation area. All analyses were performed according to methods... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coliforms; Escherichia coli; Food; Microbiological quality; Oysters. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200012 |
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| Yamamoto,K.; Matsubara,M.; Kawasaki,S.; Bari,M.L.; Kawamoto,S.. |
| Escherichia coli, as a model microorganism, was treated in phosphate-buffered saline under high hydrostatic pressure between 100 and 300 MPa, and the inactivation dynamics was investigated from the viewpoint of predictive microbiology. Inactivation data were curve fitted by typical predictive models: logistic, Gompertz and Weibull functions. Weibull function described the inactivation curve the best. Two parameters of Weibull function were calculated for each holding pressure and their dependence on holding pressure was obtained by interpolation. With the interpolated parameters, inactivation curves were simulated and compared with the experimental data sets. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Predictive microbiology; Pressure; Escherichia coli; Inactivation; Model. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800014 |
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| Bennemann,Paulo Eduardo; Bortolozzo,Fernando Pandolfo; Wentz,Ivo; Cardoso,Marisa Ribeiro de Itapema. |
| Foram utilizados 12 ejaculados, coletados de maneira asséptica, distribuídos em sete tratamentos, sendo um grupo controle e os demais inoculados com três diferentes concentrações de S. aureus ou E. coli (5 x 10(5), 5 x 10(6) e 5 x 10(7) UFC/ml). Durante 96 horas, foram avaliados a motilidade espermática, o percentual de acrossomas intactos (NAR), o pH e o número de unidades formadoras de colônia (UFC/ml) das bactérias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com exceção do tratamento com a inoculação de 5 x 10(7) UFC de E. coli/ml, não foi observado efeito significativo das bactérias sobre a motilidade espermática (p>0,05). Da mesma forma, não houve efeito significativo (p>0,05) do S. aureus ou... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sêmen; Acrossoma; Escherichia coli; Staphylococcus aureus. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000200019 |
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| Santo,Edilene; Salvador,Miriam Mendonça; Marin,José Moacir. |
| Multiple resistances to antimicrobial drugs arising in Escherichia coli isolates may complicate therapeutic management of urinary tract infection (UTI) by this organism. In order to assess the multidrug resistance (MDR) among urinary E. coli isolates, we have tested 11 antimicrobial drugs against 67 isolates from outpatients attended in a tertiary-care teaching hospital and of 78 isolates from a municipal health unit, respectively in Ribeirão Preto, State of São Paulo, Brazil. Seventy-six percent and 22% of the isolates from the tertiary-care hospital and the municipal unit, respectively, were resistant to three or more different classes of agents, and were considered to present MDR. Among the isolates from the hospital patients, 73.0%, 65.0%, 58.0%, 58.0%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Urinary tract infections; Escherichia coli; Antimicrobial susceptibility; Multidrug-resistance. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000600010 |
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| Le Guyader, Soizick; Loisy, Fabienne; Atmar, R; Hutson, A; Estes, M; Ruvoen-clouet, N; Pommepuy, Monique; Le Pendu, J. |
| The primary pathogens related to shellfishborne gastroenteritis outbreaks are noroviruses. These viruses show persistence in oysters, which suggests an active mechanism of virus concentration. We investigated whether Norwalk virus or viruslike particles bind specifically to oyster tissues after bioaccumulation or addition to tissue sections. Since noroviruses attach to carbohydrates of the histo-blood group family, tests using immunohistochemical analysis were performed to evaluate specific binding of virus or viruslike particles to oyster tissues through these ligands. Viral particles bind specifically to digestive ducts (midgut, main and secondary ducts, and tubules) by carbohydrate structures with a terminal N-acetylgalactosamine residue in an alpha... |
| Tipo: Text |
Palavras-chave: Bioaccumulation; Shellfish; Escherichia coli; Pathogen; Oyster digestive tissue; Noroviruses. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1542.pdf |
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| Registros recuperados: 237 | |
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